|COOKIES: By using this website you agree that we can place Google Analytics Cookies on your device for performance monitoring.|
Circulating tumour DNA as a biomarker of genetics alterations in cancer
If you have a question about this talk, please contact Florian Markowetz.
Circulating tumour DNA as a biomarker of genetics alterations in cancer. Application to the quantitative determination of KRAS and BRAF mutations in plasma of colorectal cancer patients.
The pressing need to determine the KRAS mutational status for selecting patients with colorectal cancer for anti-EGFR therapy provides a great opportunity to use circulating tumor DNA (ctDNA) as a theranostic tool. However the determination of genetic alteration in ctDNA could benefit of a better knowledge of ctDNA, especially at the structural level.
We accumulated data on ctDNA structural characteristics enabling us to develop a specific ctDNA methodology. Intplex is based on Allele Specific Blocker Q-PCR, and consequently is simple, fast, and cost effective, and can discriminate targeted point mutation from even single copies of ctDNA. KRAS and BRAF mutations are driven mutations in CRC (colorectal cancer) and affect treatment outcome. Intplex was adapted to detect seven KRAS (G12V, G12D , G13D, G12S , G12A, G12C , G12R) and one BRAF V600E point mutations. KRAS and BRAF mutational status was determined with high concordance when comparing ctDNA analysis and conventional methods from tumor tissue section, and greater sensitivity (0.01% mutant/WT ratio).
We showed that CRC plasma analysis could replace advantageously tumour-section analysis of targeted point mutation and expand the scope of personalized medicine for cancer patients.
Hosted by Nitzan Rosenfeld
This talk is part of the Seminars on Quantitative Biology @ CRUK Cambridge Institute series.
This talk is included in these lists:
Note that ex-directory lists are not shown.
Other listsCentre for Animal Welfare & Anthrozoology Seminars Royal Society Rosalind Franklin Seminar Series Biological Anthropology Easter Term Seminars 2012
Other talksThe 2015 Pregnancy Summit Characterisation of the interaction between the trypanosome haptoglobin-haemoglobin receptor and its ligands. Long term health economic modelling of screening strategies for cardiovascular disease prevention (work in progress). Inferno XXV, Purgatorio XXV, Paradiso XXV Aron’s political antinomies and the constitution of international order. DNA damage response: Mechanisms and relevance for cancer development and treatment