University of Cambridge > Talks.cam > Seminars on Quantitative Biology @ CRUK Cambridge Institute  > Allele specific expression design using short read DNA sequencing and application to the genetics of autoimmune disorders

  Browse A-Z

Log in

Cambridge users (raven)details Other usersdetails No account?details

Information on

Subscribing to talksdetails Finding a talkdetails Adding a talkdetails Disseminating talksdetails Help and Documentationdetails

Allele specific expression design using short read DNA sequencing and application to the genetics of autoimmune disorders

Add to your list(s) Download to your calendar using vCal

If you have a question about this talk, please contact Florian Markowetz.

The widespread use of genome-wide association studies has enabled the identification of a large number of disease associated genetic variants, in particular for autoimmune disease like type 1 diabetes and celiac disease. A key challenge is the design of functional assays to understand the molecular role of these risk alleles. Taking RNA as a first step, the typical expression quantitative trait locus (eQTL) experiment correlates gene expression with genotypes in large collections of samples. This design is not practical for samples that are difficult to collect on a large scale such as brain tissue or activated T cells. An alternative to measuring expression differences across individuals is to compare the relative expression of both chromosomes within the same individual. This is the principle of the allele specific expression (ASE) design. Here, I describe how high throughput DNA sequencing can improve the accuracy of ASE assays. However, this process is challenging owing to the multiple biases associated with the sequencing step. Bioinformatic tools and statistical methods need to be constructed to account for these difficulties. I then apply this approach to analyze a set of autoimmune genes.

Hosted by Ana-Teresa Maia

This talk is part of the Seminars on Quantitative Biology @ CRUK Cambridge Institute series.

Tell a friend about this talk:

This talk is included in these lists:

Note that ex-directory lists are not shown.

Other lists

history Early Science and Medicine C.U. Ethics in Mathematics Society (CUEiMS)

Other talks

The role of the oculomotor system in visual attention and visual short-term memory Developing novel methods for interrogating tree ring anatomy for use in modelling carbon sequestration TODAY Adrian Seminar - "Physiological and genetic heterogeneity in hearing loss" Autumn Cactus & Succulent Show Don't be Leeroy Jenkins – or how to manage your research data without getting your whole project wiped out THE PYE STORY Protein Folding, Evolution and Interactions Symposium Katie Field - Symbiotic options for the conquest of land Crowding and the disruptive effect of clutter throughout the visual system 'Ways of Reading, Looking, and Imagining: Contemporary Fiction and Its Optics' Genomic Approaches to Cancer Evolution’s Bite: Dental evidence for the diets of our distant ancestors
 

© 2006-2024 Talks.cam, University of Cambridge. Contact Us | Help and Documentation | Privacy and Publicity