University of Cambridge > > BSS Formal Seminars > Light sheet fluorescence microscopy for fast volumetric imaging of bio-samples

Light sheet fluorescence microscopy for fast volumetric imaging of bio-samples

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Light sheet fluorescence microscopy (LSFM) is a convenient tool for high resolution, 3D, imaging as it efficiently collects the generated fluorescence while at the same time minimizes photobleaching. Being based on an intrinsic plane illumination, it allows for a fast 2D imaging. Therefore, LSFM has been put forward as an interesting candidate for fast volumetric (3D) imaging.

I will present a LSFM microscope which is combined with the use of wavefront coding (WFC) techniques for achieving fast 3D imaging. WFC is used to extend the depth of field (DOF) of the collecting objective in a LSFM . This result in a system in which the light sheet can be axially scanned through the biological sample (which remains static). This provides LSFM with intrinsic volumetric imaging capabilities for visualising the fast dynamics in 3D of living samples such as C. elgans worms, Drosophila melanogaster fly embryos and Danio rerio zebrafish.

This talk is part of the BSS Formal Seminars series.

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