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Fluorescence Lifetime Techniques for Biomedical Applications

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This presentation overviews fluorescence lifetime spectroscopy and imaging techniques for label-free in vivo characterization of biological tissues. Emphasis is placed on recently developed devices and methods enabling real-time characterization and diagnosis of diseased tissues during clinical interventions. I will present studies conducted in animal models and human patients demonstrating the ability of lifetime fluorescence techniques to provide rapid in-situ evaluation of tissue biochemistry and their potential to guide surgical, biopsy, and intravascular procedures. Current results demonstrate that tissue intrinsic fluorescence features can provide useful contrast for diagnosis of vulnerable atherosclerotic plaques and for intraoperative delineation of brain tumors and head and neck tumors. Finally, I will present results from the first-in-human study focused on the integration of a scanning multispectral fluorescence lifetime technique with the da Vinci surgical robot and demonstrating the potential of this technique as a tool for augmented reality during trans-oral robotic surgery (TORS).

This talk is part of the BSS Formal Seminars series.

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