University of Cambridge > > Evolution and Development Seminar Series > TCF/Lef regulates the Gsx ParaHox gene in central nervous development in chordates.

TCF/Lef regulates the Gsx ParaHox gene in central nervous development in chordates.

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The ParaHox genes play an integral role in the anterior-posterior (A-P) patterning of the nervous system and gut of most animals. The ParaHox cluster is an ideal system in which to study the evolution and regulation of developmental genes and gene clusters, as it displays similar regulatory phenomena to its sister cluster, the Hox cluster, but offers a much simpler system with only three genes. Using Ciona intestinalis transgenics, we isolated a regulatory element upstream of Branchiostoma floridae Gsx that drives expression within the central nervous system of Ciona embryos. The minimal amphioxus enhancer region required to drive CNS expression has been identified, along with surrounding sequence that increases the efficiency of reporter expression throughout the Ciona CNS . TCF/Lef binding sites were identified and mutagenized and found to be required to drive the CNS expression. Also, individual contributions of TCF /Lef sites varied across the regulatory region, revealing a partial division of function across the Bf-Gsx-Up regulatory element. Finally, when all TCF /Lef binding sites are mutated CNS expression is not only abolished, but a latent repressive function is also unmasked. We examine the evolutionary and developmental implications of these results, and discuss the possibility of TCF /Lef not only as a regulator of chordate Gsx, but as a deeply conserved regulatory factor controlling all three ParaHox genes across the Metazoa.

This talk is part of the Evolution and Development Seminar Series series.

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