Multidimensional fluorescence imaging in living cells

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• Dr Clemens Kaminski (Department of Chemical Engineering, Cambridge)
• Friday 17 November 2006, 11:30-12:00
• Kaetsu Centre, New Hall.

If you have a question about this talk, please contact Duncan Simpson.

Watching Living Matter

Fluorescence microscopy techniques are ideally suited for the study of living matter. They are sensitive and non intrusive and thus can be applied in situ without disturbing the processes under investigation. This talk will focus on new developments in the Laser Analytics Group of techniques offering multidimensional and quantitative information from live cell systems. We focus not only on the intensity of emitted fluorescence light but also on the spectrum and lifetime. Variants of these techniques permit information to be obtained on spatial scales below the wavelength of the exciting light. Examples will be shown from studies of cell topology changes upon parasitic infection, protein-protein interactions, and the delivery of macromolecular complexes to live cell systems.

This talk is part of the Physics of Living Matter PLM6 series.

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