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The yeast Pif1 family helicase RRM3 promotes DNA unwinding during replisome swivelling

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Topological Dynamics in the Physical and Biological Sciences

During termination of DNA replication, replisomes overcome the topological tension that occurs as forks converge by coupling final unwinding with fork swivelling. Here I show that cells lacking the DNA helicase RRM3 accumulate terminating late replication intermediates (LRI) in plasmids both with and without characterised pause sites. Rrm3 deletion does not alter the level of swivelling that occurs during termination but its depletion extends the lifetime of LRI while its over-expression leads to the rapid unwinding of the LRI . Therefore RRM3 promotes DNA unwinding when the replisome swivels during termination. Potentially, this activity is also generally utilised during DNA replication to bypass topological blocks.

This talk is part of the Isaac Newton Institute Seminar Series series.

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