In Vivo studies with Optical Tweezers

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• Prof. Lene B. Oddershede, Niels Bohr Institute, Univ. Copenhagen, Blegdamsvej 17, 2100 Copenhagen, Denmark
• Friday 15 January 2010, 14:00-15:00
• Pippard Lecture Theatre, Cavendish Laboratory.

If you have a question about this talk, please contact Erika Eiser.

Optical tweezers are a very versatile nano-tool capable of measuring forces and distances inside living cells in a nearly non-invasive manner. In order to use the technique to probe fundamental physical characteristics of living cells it is crucial that the tweezers do not influence the physiological state of the investigated organism¹, and that the potential temperature increase associated with optical trapping is not significant. Both issues will be addressed in the talk, the latter using a novel lipid bilayer molecular sorting assay. Optical investigations of two different biological systems will be presented: The motility of an individual protein in the outer membrane of E. coli bacteria will be unraveled, the observed mobility being caused not only by thermal motion both in addition by an active motion associated with the metabolism of the organism [2,3]. Connected to this, we show that antibiotics and antimicrobial pep- tides have a pronounced effect on single protein motility [4]. The second biological system presented will be an S. pombe yeast cell, where the diffusion patterns of naturally occurring lipid granules have been uncovered using optical trapping and single particle tracking; the granules perform anomalous diffusion, with subdiffusion being most predominant at short time-lags, and the biological functions giving motility footprints at longer time-lags [5,6].

1. M.B. Rasmussen, L.B. Oddershede, H. Siegumfeldt, Applied and Environmental Microbiology, 74 (2008). 2. L. Oddershede, J.K. Dreyer, S. Grego, S. Brown, K. Berg-Sørensen, Biophysical Journal, 83, 3152 (2002). 3. T. Winther, L. Xu, K. Berg-Srensen, S. Brown, and L.B. Oddershede. Biophysical Journal, Bio-physical Journal, 97, 1 (2009). 4. T. Winther and L.B. Oddershede, Current Pharmaceutical Biotechnology,10, 486-493 (2009). 5. I.M. Tolic-Nørrelykke, E.-L. Munteanu, G. Thon, L. Oddershede, and K. Berg-Sørensen, PRL , 93, 078102 (2004). 6. V. Tejedor, O. Benichou, R. Voituriez, R. Jungmann, F. Simmel, C. Selhuber-Unkel, L.B. Odder- shede, and R. Metzler, Biophysical Journal (2010).

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