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Optically-assisted interrogation of cells

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If you have a question about this talk, please contact Jurij Kotar.

Laser light has many applications in the life sciences ranging from imaging of cells in culture to disease therapy in humans. I shall present some work in the field of biophotonics based upon optical trapping, the technique in which a focused laser beam is used to pick up and move transparent, microscopic particles. I will describe how optical micromanipulation and microdissection of chromosomes are used to create fluorescent in situ hybridisation (FISH) probes to detect changes in the DNA of chromosomes and how we use optical patterns, such as the Bessel beam or interference patterns, for cell sorting applications. I will go on to discuss the laser-assisted delivery of particles into single cells (including photoporation) and the optical manipulation of particles inside living cells (in cyto) including the applications and limitations of this tool.

This talk is part of the BSS Formal Seminars series.

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