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Watching and modelling limb development

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Watching Living Matter

While time-lapse imaging techniques have proven themselves to be invaluable in understanding vertebrate organogenesis, they have tended to focus on detailed qualitative observations of groups of cells within the organ in question. By contrast, despite its likely benefits to computer modelling, quantitative mapping of the global behaviour of an entire organ has often been neglected. We have therefore been developing a new 4D imaging technique – time-lapse OPT – which aims to capture the physical dynamics and changing gene expression patterns of an entire growing mouse limb bud. This type of data will be used to improve our computer simulations of limb morphogenesis.

This talk is part of the Physics of Living Matter PLM6 series.

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