University of Cambridge > > Plant Sciences Research Seminars > Plasma membrane electron transport in the green alga Chlamydomonas reinhardtii

Plasma membrane electron transport in the green alga Chlamydomonas reinhardtii

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Biophotovoltaic (BPV) bioenergy devices capture electrons extruded by single-celled algae and convert these into a power current. Mechanisms of electron extrusion in algae are unclear, though the plasma membrane (PM) is a putative barrier. Chlamydomonas reinhardtii is being used to investigate this process. C.reinhardtii harbours two key PM electron transport candidates: iron reductases and NADPH oxidases (NOX). NOX proteins generate superoxide anion (O2.) by transporting cytosolic electrons to apoplastic oxygen. An assay was developed to measure O2. production. O2.- production by C.reinhardtii appears to be sensitive to known NOX inhibitors and is light-dependent. In addition, the biological function of the C.reinhardtii NOX is being elucidated using RT-PCR. Bioinformatics analyses, NOX assay development, and RT-PCR will be discussed, with an emphasis on using these to investigate PM electron transport.

This talk is part of the Plant Sciences Research Seminars series.

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