Molecular Flexibility in Cryo Electron Microscopy

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• Johannes Schwab (MRC Laboratory of Molecular Biology)
• Thursday 18 May 2023, 16:00-17:00
• Seminar Room 1, Newton Institute.

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RNTW03 - New tomographic methods using particles

Cryogenic electron microscopy (cryo-EM) is a powerful technique toobtain the 3D structure of macromolecules from thousands of noisyprojection images. Since these macromolecules are flexible by nature,the areas shows lower resolution and gives a blurry reconstruction. Wepropose a novel method incorporated in a software package nameddynamight, that represents the molecule with gaussian basis functionsand estimates deformation fields for every experimental image by avariational autoencoder. We further use the estimated deformations tobetter resolve the flexible regions in the reconstruction using afiltered backprojection algorithm along curved lines. We presentresultson real data showing that we obtain improved 3D reconstruction.

This talk is part of the Isaac Newton Institute Seminar Series series.

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