Spatial requirements for T-cell receptor triggering probed via a DNA origami-based biointerface

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• Dr Eva Sevcsik, Institute of Applied Physics, TU Wien
• Monday 10 May 2021, 14:30-15:30
• Zoom: https://ceb-cam-ac-uk.zoom.us/j/89685956990?pwd=dTJ4am8vRFBGYzMvRDBuNnUrVkNtZz09.

If you have a question about this talk, please contact Francesca van Tartwijk.

The nanoscale spatial organization of ligands and receptors is emerging as an important theme in regulating cell behavior yet inherently challenging to investigate. Antigen recognition by T-cells illustrates this conundrum: while central to adaptive immunity and with most molecular players already identified, knowledge on its operational principles is still limited. We have devised a DNA origami-based biointerface which allows the experimenter to adjust protein distances with nanometer precision as a means to enhance or disturb signaling while being responsive to large scale reorganization processes during cell activation. Applying this biointerface to study the spatial requirements of T-cell activation we find that the smallest signaling-competent receptor unit consists of two stably ligated T-cell receptors (TCRs) within a distance of 20 nanometers. Spatial organization of the physiological ligand pMHC, however, is not a relevant parameter of antigen-mediated T-cell activation as single, well-isolated pMHC molecules efficiently stimulate T-cells.

This talk is part of the Laser Analytics Group Seminars series.

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• Zoom: https://ceb-cam-ac-uk.zoom.us/j/89685956990?pwd=dTJ4am8vRFBGYzMvRDBuNnUrVkNtZz09

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