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SUMMARY:Spatial requirements for T-cell receptor triggering probed via a D
 NA origami-based biointerface - Dr Eva Sevcsik\, Institute of Applied Phys
 ics\, TU Wien
DTSTART:20210510T133000Z
DTEND:20210510T143000Z
UID:TALK160408@talks.cam.ac.uk
CONTACT:Francesca van Tartwijk
DESCRIPTION:The nanoscale spatial organization of ligands and receptors is
  emerging as an important theme in regulating cell behavior yet inherently
  challenging to investigate. Antigen recognition by T-cells illustrates th
 is conundrum: while central to adaptive immunity and with most molecular p
 layers already identified\, knowledge on its operational principles is sti
 ll limited. We have devised a DNA origami-based biointerface which allows 
 the experimenter to adjust protein distances with nanometer precision as a
  means to enhance or disturb signaling while being responsive to large sca
 le reorganization processes during cell activation. Applying this biointer
 face to study the spatial requirements of T-cell activation we find that t
 he smallest signaling-competent receptor unit consists of two stably ligat
 ed T-cell receptors (TCRs) within a distance of 20 nanometers. Spatial org
 anization of the physiological ligand pMHC\, however\, is not a relevant p
 arameter of antigen-mediated T-cell activation as single\, well-isolated p
 MHC molecules efficiently stimulate T-cells.
LOCATION:Zoom: https://ceb-cam-ac-uk.zoom.us/j/89685956990?pwd=dTJ4am8vRFB
 GYzMvRDBuNnUrVkNtZz09
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