University of Cambridge > Talks.cam > Isaac Newton Institute Seminar Series > A novel biophysical method for the study of tethered signalling reactions

A novel biophysical method for the study of tethered signalling reactions

Add to your list(s) Download to your calendar using vCal

If you have a question about this talk, please contact info@newton.ac.uk.

SDB - Stochastic Dynamical Systems in Biology: Numerical Methods and Applications

Many signalling reactions depend on the signalling protein first localising near its substrate before catalysing a reaction. A common example is the binding of cytosolic enzymes to the unstructured cytoplasmic tails of immune receptors. The tethering (binding) of these enzymes to the tails of immune receptors influences the local concentration of substrate that they experience. In contrast to cytosolic reactions, we currently do not have experimental tools to study tethered signalling reactions. In this work-in-progress talk, I will present an experimental assay that we have been using to study tethered signalling. I will show that the PDE model fails to fit the data (which is averaged over moles of protein) whereas the equivalent stochastic simulation can fit the data. We derived a modified PDE model based on a pair density formalism that can fit the data and the stochastic simulation. Ultimately, we are able to recover 4 parameters that characterise a tethered signalling reaction from the data: binding rates, catalytic rate, and a parameter that determines the properties of the tether.

This talk is part of the Isaac Newton Institute Seminar Series series.

Tell a friend about this talk:

This talk is included in these lists:

Note that ex-directory lists are not shown.

 

© 2006-2017 Talks.cam, University of Cambridge. Contact Us | Help and Documentation | Privacy and Publicity