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In search of the brain's wiring

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Although biological imaging techniques have seen vast improvements over the past century, there is still no method capable of reconstructing the whole neuronal circuitry in a tissue sample; information that is direly needed for a better understanding of how the brain works. Serial Block-Face Scanning Electron Microscopy (SBFSEM), developed by W. Denk and H. Horstmann in 2004, promises to finally provide an answer to this central question of neuroscience. Yet interpreting the data proves to be a challenge for signal reconstruction techniques.

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