RNA Genomics in Health and Disease

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• Robert B Darnell, MD PhD, The Rockefeller University and Howard Hughes Medical Institute
• Thursday 12 April 2018, 13:00-14:00
• CRUK CI Lecture Theatre.

If you have a question about this talk, please contact Kate Davenport.

Cell-specific RNA CLIP

Our laboratory has discovered that neurons have unique systems for regulating RNA metabolism that are used by neurons. These neuron-specific RNA binding proteins are encoded by multigene families that encode such regulatory factors as Nova, nElavl, Mbnl and RbFox (NeuN). We developed the CLIP method to precisely map RNA -protein interaction sites in living brain or other tissues with single nucleotide resolution. This has led to new understanding of the function of these proteins in vivo. CLIP is a general method, and we have expanded it to the study of the Fragile-X protein FMRP and Argonaute-microRNA-mRNA ternary interactions, allowing identification of translational control and microRNA binding sites on a transcriptome-wide scale. We will discuss more recent explorations of CLIP in functional annotation of the transcriptome, and a new platform method to undertake CLIP studies in single cell types within the brain. The increasing resolution of CLIP allows new insights into cell-specific roles of RNA regulation in specific paradigms of normal and abnormal cellular physiology.

Select References (past decade) Licatalosi et al. Nature 456:464-9, 2008; Chi et al., Nature 460:479-86, 2009; Zhang et al., Science 329:439-443, 2010, Darnell et al., Cell 146:247-61, 2011; Ince-Dunn et al., Neuron 75:437-450, 2012; Darnell, Ann Rev Neurosci 36:243-70, 2013; Vanharanta et al., eLife 4:e02734, 2014; Moore et al., Nature Comm 6:8864-81, 2015; Luna et al., Cell 160:1099-1110, 2015; Mol Cell 67:400-410, 2017; Hwang et al., Cell Reports 15:423-435, 2016; Neuron 95:1334-1349, 2017; Jereb et al., eLife, 2018 Mar 26;7. pii: e34042; Yuan Y et al., Darnell RB. BioRxiv 237347; doi: https://doi.org/10.1101/237347, 2018.

This talk is part of the Cancer Research UK Cambridge Institute (CRUK CI) Seminars in Cancer series.

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