Using the auxin-inducible degron in yeast to understand the spliceosome cycle

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• Gonzalo Mendoza, Smith Lab
• Thursday 18 January 2018, 13:00-13:30
• Department of Plant Sciences, Large Lecture Theatre.

If you have a question about this talk, please contact Pallavi Singh.

Pre-mRNA splicing is essential for eukaryotic cells and is catalyzed by a very large RNA /protein particle called the spliceosome. During my PhD, my initial research aim was to identify proteins potentially involved in linking splicing with transcription. To this end, I used the auxin-inducible degron technique to quickly deplete proteins of interest, and chromatin immunoprecipitation to measure in vivo assembly of the spliceosome. I discovered that knocking-down splicing proteins can quickly lead to strong secondary effects that can mask the primary consequence of the depletion. I will explain where these secondary effects are coming from, suggest one way to avoid them and discuss the implications of this finding. Towards the end of my talk, I will present the plans I have of applying pre-mRNA splicing techniques to engineer the thiamine-responsive riboswitch of Chlamydomonas reinhardtii. My goal is to create novel molecular tools to control transgene expression in plants.

This talk is part of the Plant Sciences Departmental Seminars series.

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