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Single Molecule Enzymology: Transcription against Supercoiling

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Single molecule enzymology has yielded the mechanistic understanding of enzymes both in vitro and in live cells. Because DNA exists as single molecules that carry genetic codes in an individual cell, gene expression is stochastic. Recent single molecule studies of gene expression in live cells have allowed the quantitative description of the central dogma of molecular biology. The molecular mechanism of the ubiquitous phenomenon of stochastic transcription, the burst production of mRNA, has been revealed by single molecule enzymology.

This talk is part of the Chemistry Departmental-wide lectures series.

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