Chlamydomonas reinhardtii NADPH Oxidases as potential electron transporters for biophotovoltaic devices.

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• Xander Anderson
• Thursday 21 June 2012, 16:30-17:00
• Department of Plant Sciences, Large Lecture Theatre.

If you have a question about this talk, please contact Suzy Stoodley.

Biophotovoltaic devices can harvest electrons from the outer surface (i.e plasma membrane) of algal cells (such as Chlamydomonas) and use these to generate power. NADPH Oxidases (NOX) are plasma membrane enzymes that transport electrons from intracellular NADPH to extracellular oxygen, generating superoxide anion. In other algae, NOX are important for grow and toxicity, and NOX are the primary electron transporters for biophotovoltaic devices constructed with animal cells. C.reinhardtii harbours two putative NOX (RBOL1 and 2) which I am investigating as potential plasma membrane electron transporter mechanisms. In the talk I will discuss NOX sequence analysis, measurements of NOX activity and gene expression. I will also outline molecular techniques, such as amiRNA silencing, promoter driven overexpression and GFP localisation, which I am using to understand both the biological function of these NOX in C.reinhardtii and the potential role they play as electron sources for biophotovoltaic devices.

This talk is part of the Plant Sciences Research Seminars series.

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