Seeing memories through the looking glass: hippocampal circuit activity in mice performing spatial and working memory tasks

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• Prof. Simon R. Schultz, Centre for Neurotechnology, Imperial College London
• Wednesday 10 May 2023, 10:30-11:30
• Department of Chemistry, Unilever Lecture Theatre.

If you have a question about this talk, please contact Alyssa Miller.

We have recently demonstrated the use of an air-lifted behavioural platform that is compatible with two-photon microscopy to map place fields, using the CA1 hippocampal network as a rich and effective memory readout (Go et al, Front Cell Neurosci 15:19, 2021). This enables us to examine the spatiotemporal dynamics of neural activity as memories are laid down, and as they are later recalled. I will describe how we have employed this system to characterise degradation in memory performance in transgenic mice (5xFAD mice, which over-express mutant human amyloid precursor protein) performing a circular track memory task, allowing us to quantify effects on neural ensemble activity in both memory recall and encoding. In further work, we have used this platform to study changes in network function in 5xFAD mice performing a forced alternation spatial working memory task. We see effects related to amyloidosis in both degradation of spatial information in the hippocampus, and in performance on the alternation task. Finally, I will discuss the pipeline we have been developing to track the effects of neurodegenerative disease in the same animal through behaviour, in vivo multiphoton imaging from targeted brain regions, and finally ex vivo whole-brain two-photon tomography registered to the Allen Brain Atlas. If there is time, I will also introduce new work on two-photon mesoscopy, and computational neuroscience work on neural manifold approaches for analysing large-scale neural datasets.

This talk is part of the Biophysical Seminars series.

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