Identification of Potential CCM Candidates in Chlamydomonas reinhardtii

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• Dr Indu Santhanagopalan, Physiological Ecology Group, Department of Plant Sciences
• Thursday 17 February 2022, 13:00-14:00
• Online.

If you have a question about this talk, please contact Kumari Billakurthi.

The Carbon Concentrating Mechanism (CCM) in green algae is a biophysical pump delivering high concentrations of intracellular carbon dioxide to enhance the fixation of carbon by CBB cycle. This is achieved with a large number of transporters for uptake of carbon dioxide and bicarbonate ions, strategically placed carbonic anhydrases, and compartmentalization of rubisco enzyme, concentrated in the pyrenoid microcompartment.

In this talk, I shall be describing the identification of potential CCM genes by analysis of expression networks in the green alga Chlamydomonas reinhardtii. Screening of knockouts for the identified potential CCM genes led us to two new candidates with unexpected phenotypes. The two mutants- a putative ABC transporter called MRP2 , and a gene encoding a protein with EF- hand domain identified; have an unexpected, enhanced carbon concentration, and grow better than wild type under carbon limiting conditions. While the exact role and mechanism of the two genes in CCM remains to be elucidated, physiological measurements and experiments carried out with the two mutants suggest differences in the inorganic carbon transport mechanisms.

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