University of Cambridge > > Biophysical Seminars > ApoE, Ab and Alzheimer’s disease: From test tube to transgenic mice

ApoE, Ab and Alzheimer’s disease: From test tube to transgenic mice

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If you have a question about this talk, please contact Alyssa Miller.

Seminar Outline (Major concepts to be discussed):

  • Alzheimer’s disease (AD) is the most common cause of dementia. While rare, familial AD is caused by genetic mutations that increase the production of Ab42.
  • Age, APOE genotype and sex are the universal biological variables (UBV) of AD risk.
SDS -stable apoE/Ab complexes are apoE isoform specific (apoE3/Ab > apoE4/Ab).
  • Biochemical detection
  • In vitro function
Ab42 oligomers and fibrils
  • Biochemical assembly
  • In vitro function
The development of reagents for in vivo studies:
  • Optimization of a 3-step sequential protein extraction
  • Characterization of a new Ab-specific monoclonal antibody
  • Development of oligomer-specific ELISA
  • Generation of the EFAD transgenic mouse model

The EFAD transgenic mice develop AD-relevant pathology that increases significantly with age, APOE4 and female sex, mirroring human AD risk.

This talk is part of the Biophysical Seminars series.

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