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Watching single molecules

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If you have a question about this talk, please contact Dr Eileen Nugent.

For about the last 15 years it has been possible to directly observe individual molecules in solution, attached to surfaces or in the membrane of live cells. My group has focussed on developing these methods and applying them to biological or biomedical problems, which have been difficult or impossible to solve using more conventional bulk methods. I will firstly introduce scanning ion conductance microscopy, which allows nanoscale imaging of live cells and controlled delivery of molecules to the cell surface. Next I will show how we have developed single molecule fluorescence to detect the interaction of molecules in the test-tube and on cells. I will then show how these methods can be used to gain new molecular insights by describing our recent work on T-cell triggering and the role of proteins aggregates in neurodegenerative disease.

This talk is part of the BSS Formal Seminars series.

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