Creating predictable gene knock-outs at scale with CRISPR/Cas9

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• Dr. Luca Crepaldi (Sanger) & Dr. Felicity Allen (Sanger)
• Wednesday 06 March 2019, 18:30-20:00
• Postdoc Centre, Biomedical Campus, Bay 13, Hills Road, Cambridge CB2 0SP.

If you have a question about this talk, please contact Postdoc Masterclass.

CRISPR /Cas9, a recently discovered DNA editing system, is revolutionising biological research across medicine, agriculture and fundamental cell biology. It is employed to disable a selected gene to test whether this affects a particular cell function, such as development or cancerous growth. Large-scale experimental designs allow gene inactivation to be carried out in pools of human or other eukaryotic cell lines, such that each and every gene is tested simultaneously, in a comprehensive, unbiased fashion. The first speaker will: • Give an introduction to CRISPR /Cas9 and how it works • Explain how CRISPR /Cas9 can be used at scale to screen for genes relevant to a biological function The second speaker will: • Describe research examining and predicting the precise DNA mutations induced by CRISPR /Cas9 • Outline how this can be used to produce better gene knock-outs and effective genetic screens

This talk is part of the "Life Sciences Masterclass" series.

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