Functions of Dynamin A, Myosin IB and WASH in phagolysosome biogenesis, and the Exocyst in kiss-and-run exocytosis in Dictyostelium

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• Thierry Soldati, University of Geneva
• Monday 23 January 2012, 16:15-18:00
• Max Perutz Lecture Theatre, Medical Research Council (MRC) (MRC Laboratory of Molecular Biol.

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Biological processes such as motility and phagocytosis (1) are conserved and thus, their study in the common amoeba Dictyostelium discoideum, a biochemically and genetically tractable professional phagocyte, is directly relevant to higher organisms. In recent years, it has become a popular model system to study the involvement of the actomyosin cytoskeleton in vesicle formation and transport in particular during phagolysosome biogenesis. For example, class I myosins play a variety of roles during phagocytic uptake, membrane retrieval, phagosome reneutralisation and exocytosis (2). At every stage, they collaborate with a variety of other molecular machineries, including actin, the WASH complex and Dynamin A (3, 4, 5). In addition, preparation for exocytosis involves the conserved Exocyst complex. The involvement or Rab8a-mediated Exocyst function was best studied during the kiss-and-run discharge of the contractile vacuole system. In conclusion, our investigations of molecular mechanisms of membrane trafficking in Dictyostelium continue to unravel novel roles for many highly studied conserved proteins.

(1) Boulais, J., Trost, M., Landry, C., Dieckmann, R., Levy, E., Soldati, T., Michnick, S., Thibault, P., and Desjardins. M. Molecular characterization of the evolution of phagosomes. Mol. Syst. Biol. (2010) 6:423 (2) Soldati, T., and Schliwa, M. Powering membrane traffic in endocytosis and recycling. Nat. Rev. Mol. Cell Biol. (2006) 7, 897-908. (3) Gopaldass, N., Patel, D., Kratzke, R., Dieckmann, R., Hausherr, S., Hagedorn, M., Neuhaus, E.M., Hoffman, E., Hille, K., Kuznetsov, S., and Soldati, T. Dynamin A, Myosin IB and Abp1 couple phagosome maturation to F-actin binding. Traffic (2011) in press. (4) Carnell, M., Zech, T., Calaminus, S., Ura, S., Hagedorn, M., Johnston, S., May, R., Soldati, T., Machesky L., and Insall R. Actin polymerization driven by WASH causes V-ATPase retrieval and vesicle neutralization before exocytosis J. Cell Biol. (2011) 193(5):831-9. (5) Dieckmann, R., von Heyden, SY, Kistler, C., Gopaldass, N., Hausherr, S., Crawley, S.W., Schwarz, E, Diensthuber, R.F., Côté, G.P., Tsiavaliaris, G., and Soldati, T. A myosin IK-Abp1-PakB circuit acts as a switch to regulate phagocytosis efficiency. Mol. Biol. Cell (2010) 21(9):1505-1518.

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