The evaluation of lyssavirus cross-neutralising antibody responses using lentiviral pseudotypes.

Add to your list(s) Download to your calendar using vCal

• Dr Edward Wright, University College, London
• Tuesday 11 March 2008, 11:00-12:00
• Lecture Theatre 2.

If you have a question about this talk, please contact Prof. Jonathan Heeney.

We have analysed cross-neutralisation between rabies virus, CVS -11, and two European bat lyssaviruses, EBLV -1 and -2, using lentiviral pseudotypes as antigen vectors. This development of a robust microassay that can be performed outside of high containment facilities will aid on-going surveillance and vaccine studies. Tests on blinded sera from rabies virus vaccine recipients revealed that the CVS -11 pseudotype assay had 100% concordance with the current “gold standard” fluorescent antibody virus neutralisation test, and neutralising titres strongly correlated. Pseudotypes incorporating the G-protein from different lyssavirus genotypes were used to analyse antibody titres with respect to G-protein identity. The results revealed that the relative neutralisation titres correlated broadly with the degree of G-protein diversity.

This talk is part of the Special Veterinary Medicine Seminars series.

This talk is included in these lists:

• All Talks (aka the CURE list)
• Biology
• Cambridge Immunology
• Cambridge Infectious Disease
• Cambridge Infectious Diseases
• Lecture Theatre 2
• Life Sciences
• Life Sciences
• ME Seminar
• Special Veterinary Medicine Seminars
• my_list
• other talks

Note that ex-directory lists are not shown.