3-D cryo-EM in biology: specimen preparation and imaging for tomography

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• Michael Marko
• Friday 26 May 2006, 14:15-15:15
• IRC in Superconductivity Seminar Room, Cavendish Laboratory.

If you have a question about this talk, please contact Duncan Simpson.

Electron tomography of frozen-hydrated specimens, without chemical fixation, staining, or dehydration, allows us to study the 3-D structure of cells and tissue in a near-native state. Preparation and 3-D imaging of such specimens is challenging. Developments in the application of cryo-ultramicrotomy and cryo-FIB, as well as TEM imaging using zero-loss energy filtration and Zernike phase contrast, will be discussed.

This talk is part of the BSS Formal Seminars series.

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