ApoE, Ab and Alzheimer’s disease: From test tube to transgenic mice

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• Mary Jo LaDu, University of Illinois at Chicago
• Wednesday 13 October 2021, 16:00-17:00
• https://zoom.us/j/92564124601.

If you have a question about this talk, please contact Alyssa Miller.

Seminar Outline (Major concepts to be discussed):

Background:

• Alzheimer’s disease (AD) is the most common cause of dementia. While rare, familial AD is caused by genetic mutations that increase the production of Ab42.
• Age, APOE genotype and sex are the universal biological variables (UBV) of AD risk.

SDS -stable apoE/Ab complexes are apoE isoform specific (apoE3/Ab > apoE4/Ab).

• Biochemical detection
• In vitro function

Ab42 oligomers and fibrils

• Biochemical assembly
• In vitro function

The development of reagents for in vivo studies:

• Optimization of a 3-step sequential protein extraction
• Characterization of a new Ab-specific monoclonal antibody
• Development of oligomer-specific ELISA
• Generation of the EFAD transgenic mouse model

The EFAD transgenic mice develop AD-relevant pathology that increases significantly with age, APOE4 and female sex, mirroring human AD risk.

This talk is part of the Biophysical Seminars series.

This talk is included in these lists:

• All Talks (aka the CURE list)
• Biophysical Seminar Series 2016/17
• Biophysical Seminars
• Department of Chemistry
• Featured lists
• School of Physical Sciences
• https://zoom.us/j/92564124601

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