Fluorescence recovery after photobleaching (FRAP) – from data acquisition to results interpretation

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• Natalia Bulgakova (Gurdon Institute)
• Wednesday 18 March 2015, 18:30-20:00
• Gurdon Institute Tea Room.

If you have a question about this talk, please contact YS.

Fluorescence recovery after photobleaching (FRAP) is widely used to study dynamics of proteins and protein complexes in living cells. In this Masterclass, I will start by giving an overview of the FRAP technique. Then, I will discuss the technical considerations involved in FRAP experiments, common approaches to interpret the results, and available tests to validate the interpretations. Finally, I will demonstrate examples of how FRAP was used to investigate protein dynamics in a range of biological systems.

This talk is part of the "Life Sciences Masterclass" series.

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