New tools for NGS libraries: Long-Span, Mate-Pair Scaffolding and Methods for Faster Library Creation

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• David Mead, Founder and Chief Executive Officer of Lucigen
• Wednesday 13 March 2013, 12:30-13:30
• CRUK Cambridge Institute Room 215.

If you have a question about this talk, please contact Laura Blackburn.

Places are limited, please e-mail saphsa.codling@cruk.cam.ac.uk if you want to attend

Large insert mate-pair reads have a major impact on the overall success of de novo genome assembly and the discovery of inherited and acquired structural variants. Molecular tools that bridge the gap between massively parallel short read sequencing technologies (35-1,500 bases) and the need for large scaffolds (>40,000 bases) to accurately assemble complex repeat-rich genomes are required. The NxSeq™ 40 kb Mate-Pair Cloning Kit has been developed to facilitate genome assembly and gap closure. Results show that approximately 40 kb paired-end sequences can be obtained by either Illumina or 454 sequencing at an overall efficiency of 70%, which is significantly better than existing long-span, mate-pair systems. This presentation will describe the unique pNGS FOS vector, which contains primer binding sites for next gen sequencing from Illumina or Roche 454 platforms, as well as the methodology with which to achieve long-span mate-pair libraries for de novo genome assembly. In addition, the NxSeq DNA Sample Prep Kits will be described, which feature a combined end-repair and A-tailing master mix to enable significantly faster DNA library prep for NGS applications.

Dr. Mead will be joined by representatives of Cambridge BioScience Limited; Lara Spence, Ella Popowicz and Mike Kerins.

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