CADHERIN mediated AMIS localisation - Using mouse embryonic stem cells 3D culture as a model

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• Xuan Liang (PDN, Cambridge University)
• Monday 31 January 2022, 14:30-15:30
• Online.

If you have a question about this talk, please contact Elena Scarpa.

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ndividual cells within de novo polarising tubes and cavities must integrate their forming apical domains into a centralised apical membrane initiation site (AMIS). This is necessary to enable organised lumen formation within multi-cellular tissue. Despite the well documented importance of cell division in localising the AMIS , we have found a division-independent mechanism of AMIS localisation that relies instead on CADHERIN -mediated cell-cell adhesion. Our study of de novo polarising mouse embryonic stem cells (mESCs) cultured in 3D suggest that cell-cell adhesion directs the localisation of apical proteins such as PAR -6 to a centralised AMIS . Unexpectedly, we also found that mESC cell clusters lacking functional E-CADHERIN were still able to form a lumen-like cavity in the absence of AMIS localisation and did so at a later stage of development via a ‘closure’ mechanism, instead of via hollowing. This work suggests that there are two, interrelated mechanisms of apical polarity localisation: cell adhesion and cell division. Alignment of these mechanisms in space allows for redundancy in the system and ensures the development of a coherent epithelial structure within a growing organ.

This talk is part of the Morphogenesis Seminar Series series.

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• Graduate-Seminars
• Morphogenesis Seminar Series
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