Characterization of a Mechanical Hotspot for Cell Plate Guidance Near an Adjacent Three-Way Junction

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• Marie-Cécile Caillaud, CR CNRS
• Monday 24 January 2022, 14:30-15:30
• Online.

If you have a question about this talk, please contact Dr. Wolfram Pönisch.

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When it comes to building an organ, cells need to coordinate with each other to form a functional tissue layer. In epithelia, cells attach to each other forming at their edges. In animals, mechanical stresses and mechano-sensitive signaling pathways play a central role in the formation and maintenance of three-way junctions (3WJ), which are topologically stable structures. In walled multicellular organisms, like land plants, the coordination of the 3WJ formation during cell division, and the corresponding avoidance of 4WJ, remains poorly understood and is often pictured as a passive consequence of cell division. Here, we report the existence of two positions at a conserved distance, above and below an existing 3WJ where cell plate insertion could occur in the Arabidopsis root cortex. This phenomenon, observed in other plants, is under genetic control: In an Arabidopsis mutant sac9-3, the cell plate abnormally attaches at two equidistant positions from an adjacent 3WJ, both in the root and shoot. The number of ill-formed junctions in sac9-3 mutant increased with the intensity of turgor-induced stresses borne by cell walls. This confirms our initial hint that stress plays a role in cell junction formation. Moreover, the sac9-3 mutants feature an impaired metabolism of the PI(4,5)P2 phospholipid suggesting that lipid within the plasma membrane could play a role in the mechano-transduction pathway guiding cell plate attachment.

This talk is part of the Morphogenesis Seminar Series series.

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