Identification & characterization of CML23 and CML24 in Arabidopsis thaliana circadian clock

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• Nur Izzati Mohd Noh
• Friday 03 June 2011, 13:30-14:00
• Department of Plant Sciences, Large Lecture Theatre.

If you have a question about this talk, please contact Suzy Stoodley.

The circadian clock is important to all organisms as an internal time-keeper, which facilitates adaptations to the external environment conditions. In plants, several biological mechanisms such as leaf movement and cytosolic Ca2+ concentration are regulated by the circadian clock. In this study, I will identify the function of CML23 and CML24 , calmodulin-like proteins, in the Arabidopsis thaliana circadian clock. There are 50 CML genes in A. thaliana, encoding potential Ca2+ sensors. cml23-2cml24-4 double mutants lengthened the period of circadian leaf movement rhythms compared to wild type (Col-0), although cml23-2ml24-1 double mutants had unaltered leaf movement rhythms. These results suggested that CML23 and CML24 may have overlapping roles in regulation of the Arabidopsis central circadian oscillator. Database queries from www.asociomics.com have resulted in identification of seven putative interacting partners of CML24 . However, no results were obtained for CML23 from the same database. CML24 might interact with these putative interacting partners to indirectly regulate the intracellular processes. Protein pull down analysis will be performed to identify any potential interactions between CML24 with its interacting partners. The circadian leaf movement rhythm analysis of cpk3, cpk6 and cpk3cpk6 had show effect in term of phase shift in cpk3-1 single mutant and cpk3-1cpk6-1 double mutants, but not the period. Although the data are preliminary, these results suggest a hypothesis that CPK3 acts downstream of CML24 to control the circadian clock of A. thanliana In addition, epistatic analysis will be carried out to outline the potential points of entry for CML23 and CML24 in the circadian system. To address the epistatic relationship, cml23-2cml24-4 double mutants will be crossed with known clock loss of function mutants such as toc1-1, ztl1, abi1-1 lux, elf3-1, elf4-1, lhy and cca1-1. The circadian leaf movement rhythm analysis will be used to screen for the phenotype of the triple mutants. At the end of this study, these findings will direct future understanding of the role of CML23 and CML24 in the circadian clock regulation.

This talk is part of the Plant Sciences Research Seminars series.

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