University of Cambridge > Talks.cam > Evolution and Development Seminar Series > Mouse Hox gene enhancers analysed in cell culture; and some proposals on the significance of Hox gene collinearity

Mouse Hox gene enhancers analysed in cell culture; and some proposals on the significance of Hox gene collinearity

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Mouse Hox gene enhancer analysis has previously been conducted in embryos transgenic for Hox/lacZ reporter constructs. Here I show how at least some of these studies can be carried out in cultured HepG2 cells using Hox/luciferase reporters and a doxycycline-inducible expression system. In particular, I show the following: 1) Hoxd11 enhancer is activated by Gdf11/Smad signalling, 2) Hoxb4, b8 and a7 enhancers are activated by Cdx1 protein, and 3) Hoxc8 enhancer is activated by the synergistic action of Gdf11/Smad signalling and Cdx proteins. Hox enhancer analysis in cell culture reduces mouse usage, requires low cost and skill, permits rapid assessment of putative enhancer binding motifs, and provides abundant material for DNA /transcription factor binding studies.

This talk is part of the Evolution and Development Seminar Series series.

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