Sequencing 8 organs from a single Drosophila embryo at the time of differentiation

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• David Molnar, Systems Biology Centre and Department of Genetics
• Monday 13 April 2015, 10:00-10:45
• Part II Room, Department of Genetics, Downing Site, Tennis Court Road.

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I will talk about my PhD thesis which involved sequencing the foregut, anterior midgut, posterior midgut, hindgut, Malpighian tubules, left and right salivary gland, and ventral nerve cord of a single embryo, and about the challenges in generating the data. These involved finding a new defined medium that facilitates precise dissections even in fairly young embryos, getting more or less full length transcriptomes from samples that are coming close to the size of single mammalian cells, and then finally validation of sample origins. I will point to a few first things that we can learn from the data.

I hope that this approach will bring us nearer to the semi-automated discovery of complete transcriptional regulatory networks of organ formation.

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