Computational redesign of native enzyme active sites

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• Per Junior Greisen, Technical University of Denmark
• Tuesday 01 November 2011, 14:00-14:30
• TCM Seminar Room, Cavendish Laboratory.

If you have a question about this talk, please contact Daniel Cole.

The ability to redesign enzymes to catalyze non-cognate chemical transformations would have wide-ranging applications. We have developed a computational method for repurposing the reactivity of active site functional groups of metalloenzymes to catalyze new reactions. Using this method, we have engineered a zinc-containing murine adenosine deaminase to catalyze the hydrolysis of a model organophosphate with a catalytic efficiency kcat/Km ~104 M^-1 s^-1 after directed evolution. In the high-resolution crystal structure of the enzyme, all but one of the designed residues adopt the designed conformation. The designed enzyme efficiently catalyzed the hydrolysis of the RP-isomer of a coumarinyl analog of the nerve agent cyclosarin, and showed striking substrate selectivity for coumarinyl leaving-groups. Computational redesign of native enzyme active sites complements directed evolution methods and offers a general approach for exploring their untapped catalytic potential for new reactivities.

This talk is part of the TCM Informal Seminar Series series.

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• TCM Seminar Room, Cavendish Laboratory

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