University of Cambridge > > Seminars at the Department of Biochemistry > “TissueFAXS Cytometry - A Tool for Precision Medicine: Automated Analysis of Single Cells and the Cellular Microenvironment”

“TissueFAXS Cytometry - A Tool for Precision Medicine: Automated Analysis of Single Cells and the Cellular Microenvironment”

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Determining the in-situ immune status of diseased organs or quantify coexpression of molecules on the single-cell level has mostly been subject to visual estimation, or – at best – to manual counting for decades. Hence, experts usually had the choice of the “least of evils” between guessing and endless (manual) counting. In tumor immunology, infiltrating inflammatory cells need to be phenotypically characterized on a quantitative basis. To better understand the function of inflammatory cells in tumor development, type and number of inflammatory cells and their proximity to glandular/tumor structures have to be analyzed in-situ and correlated with disease state. Using TissueFAXS™ Cytometry the time-consuming and error-prone human evaluation of stained histological sections can be approached with an observer-independent and reproducible technology platform, offering a high degree of automation, paired with user interaction at relevant points of the analytical workflow. This platform can be applied as a means of tissue cytometry for both immunofluorescence and immunohistochemistry and thus constitutes the microscopic equivalent to flow cytometry (FACS).

Likewise FACS , TissueFAXS™ can quantify any type of molecular marker in any type of cell – but in tissue context or in adherent cell culture monolayers without the need to solubilise the cells (i.e. TissueFAXS permits analyses in-situ)!

The TissueFAXS Cytometry platform can be used in clinical multi-center studies to determine the immune response to certain drugs in-situ, measure proliferation, apoptosis, cytokine expression, signalling molecules, and others. It can do end-point assays as well as live-cell imaging and time-kinetic experiments. But TissueFAXS Cytometry also promotes tissue cytometry to a new level of quality, where complex cellular interactions can be addressed on the single-cell level but still in histological context.

This talk is part of the Seminars at the Department of Biochemistry series.

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