Identifying microRNAs conditioning strong plant anti-aphid resistance

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• Deusa Abreu, Carr Lab
• Friday 12 December 2014, 10:00-10:30
• Department of Plant Sciences, Large Lecture Theatre.

If you have a question about this talk, please contact Megan Cooper.

1st year graduate seminar

Aphids transmit cucumber mosaic virus (CMV). In Arabidopsis thaliana infection by the Fny strain of CMV induces aphid feeding deterrence; thought to promote virus transmission. The CMV 2a protein triggers feeding deterrence but another CMV gene product, the 2b RNA silencing suppressor protein, induces stronger, toxicity-based aphid resistance if expressed in transgenic plants. The stronger 2b-induced aphid resistance results from disruption of microRNA (miRNA)-regulated gene expression. Since this stronger form of resistance might be useful in plant protection, I will identify specific miRNA(s) involved in its regulation. I am using the LS CMV strain, which does not induce resistance to aphids in Arabidopsis, as a carrier for ‘decoy’ sequences that mimic miRNA targets and inhibit activity of specific Arabidopsis miRNAs. I have generated a library of LS-CMV variants carrying mimic sequences to identify miRNAs conditioning strong aphid resistance.

This talk is part of the Plant Sciences Departmental Seminars series.

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