Probing dynamic conformational changes in lipid signalling using hydrogen deuterium exchangemass spectrometry

Add to your list(s) Download to your calendar using vCal

• Dr John Burke, LMB, Cambridge U.K
• Wednesday 27 March 2013, 10:15-11:15
• Perham's seminar room.

If you have a question about this talk, please contact xyp20.

Most cellular responses to extracellular stimuli have a common component of regulation arising from selective recruitment of a network of signalling complexes to membranes.  However, studying these systems remains a daunting task.  We have made unprecedented progress in understanding these systems by applying a synthesis of hydrogen deuterium exchange mass spectrometry (HDX-MS), X-ray crystallography and FRET spectroscopy. Our application of this synthesis to phosphoinositide 3-kinase (PI3K) signalling represents one of the largest (> 200 kDa) complexes studied using DXMS , and elucidates novel aspects of PI3K regulation downstream of GPC Rs as well as a unifying mechanism of how diverse oncogenic PI3K mutants upregulate activity.

This talk is part of the Experimental and Computational Aspects of Structural Biology and Applications to Drug Discovery series.

This talk is included in these lists:

• Cambridge Infectious Diseases
• PMRFPS's
• Perham's seminar room
• Seminars at the Department of Biochemistry
• biochem seminars
• ndk22's list
• rc781
• se393's list

Note that ex-directory lists are not shown.