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Membrane biology in plant-microbe interactions
If you have a question about this talk, please contact Jill Harrison.
All plant organs are vulnerable to colonisation and molecular manipulation by microbes. In our attempts to understand the full nature of the interactions that occur between a potential pathogen and its host, we are focussing on membrane biology in plant-microbe interactions. Immune receptors constitute recognition sites to detect invading pathogens and to trigger defenses, and the activity of these receptors depends upon the dynamic membrane trafficking network. The plasma membrane receptor FLAGELLIN SENSING 2 (FLS2) confers plant immunity through perception of bacterial flagellin (flg22). Following elicitation, FLS2 is internalized into vesicles. To resolve FLS2 trafficking, we exploited quantitative high-throughput confocal imaging for co-localisation studies and chemical interference. FLS2 localises to bona-fide endosomes via two distinct endocytic trafficking routes depending on its activation status. I will present our high-throughput confocal imaging pipeline to study endosomal pathways in plants and will discuss results from our current research, which addresses the molecular components regulating FLS2 endocytosis, and the intersection between FLS2 endocytosis and flg22 signaling. To further study receptor-mediated endocytosis in plant immunity we focus on the receptors EFR and PEPR1 for bacterial EF-Tu (elf18) and the endogenous elicitor pep1, respectively, as well as the co-regulator BAK1 /SERK3. I will describe recent results demonstrating that ligand-induced internalization of FLS2 represents a conserved endocytic trafficking pathway. Altogether, high-throughput confocal imaging combined with functional studies allows us to tackle the dynamic cellular changes involved in the interaction between plants and microbes.
This talk is part of the Plant Sciences Departmental Seminars series.
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