University of Cambridge > Talks.cam > Cancer Research UK Cambridge Institute (CRUK CI) Seminars in Cancer > Lgr5 stem cells in self-renewal and cancer

  Browse A-Z

Log in

Cambridge users (raven)details Other usersdetails No account?details

Information on

Subscribing to talksdetails Finding a talkdetails Adding a talkdetails Disseminating talksdetails Help and Documentationdetails

Lgr5 stem cells in self-renewal and cancer

Add to your list(s) Download to your calendar using vCal

If you have a question about this talk, please contact wattpa.

Please note that this talk is on a Tuesday (joint seminar with the MRC-LMB)

The intestinal epithelium is the most rapidly self-renewing mammalian tissue. Lgr5 is a gene transcribed in cycling, crypt base columnar cells at the crypt base. Using lineage tracing experiments the Lgr5+ve cells were identified as the stem cells of the intestinal epithelium. Furthermore, Lgr5+ve stem cells can initiate ever-expanding organoids in vitro. The Lgr5+ve stem cell hierarchy of differentiation is maintained in these organoids. Thus, intestinal crypt-villus units can be built from a single stem cell in the absence of a non-epithelial cellular niche. Although, Lgr5 stem cells persist life-long, crypts drift toward clonality quickly. The cellular dynamics are consistent with a model in which the stem cells divide symmetrically, and stochastically adopt stem or transient amplifying cell fates after cell division. Lgr5 stem cells are interspersed between differentiated Paneth cells, which produce all essential signals for stem-cell maintenance. Co-culturing of sorted stem cells with Paneth cells dramatically improves organoid formation. Genetic removal of Paneth cells in vivo results in the concomitant loss of Lgr5 stem cells. Intestinal cancer is initiated by Wnt pathway-activating mutations in genes such as APC . Deletion of APC in stem cells, but not in other crypt cells results in neoplasia, identifying the stem cell as the cell-of-origin of adenomas. Moreover, a stem cell/progenitor cell hierarchy is maintained in stem cell-derived adenomas, lending support to the “cancer stem cell”-concept.

This talk is part of the Cancer Research UK Cambridge Institute (CRUK CI) Seminars in Cancer series.

Tell a friend about this talk:

This talk is included in these lists:

Note that ex-directory lists are not shown.

Other lists

Major Public Lectures in Cambridge The Kate Pretty Lecture International Women's Week at Wolfson

Other talks

The Gopakumar-Vafa conjecture for symplectic manifolds Surface meltwater ponding and drainage causes ice-shelf flexure Anthropological engineering and hominin dietary ecology A continuum theory for the fractures in brittle and ductile solids Polynomial approximation of high-dimensional functions on irregular domains The Digital Doctor: Hope, Hype, and Harm at the Dawn of Medicine’s Computer Age Horizontal transfer of antimicrobial resistance drives multi-species population level epidemics Amino acid sensing: the elF2a signalling in the control of biological functions EU LIFE Lecture - "Histone Chaperones Maintain Cell Fates and Antagonize Reprogramming in C. elegans and Human Cells"
 

© 2006-2024 Talks.cam, University of Cambridge. Contact Us | Help and Documentation | Privacy and Publicity