Pararetroviruses (EPRV)-Derived 22nt sRNAs & Their Role In DICER2-Mediated Viral Immunity In Tomato Hybrids

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• Pei Qin -Sabrina- Ng (Baulcombe Lab Group, DPS)
• Thursday 28 November 2024, 12:15-13:45
• Department of Plant Sciences, Large Lecture Theatre.

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Abstract DICER2 -dependent 22nt small RNAs are essential in plant antiviral defence through RNA silencing. These RNAs, derived from sources such as transposable elements like endogenous pararetroviruses (EPRVs), are pivotal in enhancing plant immunity. EPR Vs, integrated into plant genomes from past infections, act as an “infection memory,” improving resistance to pararetroviruses. Our recent study shows that EPRV -derived small RNAs (sRNAs) are activated in tomato hybrids, with an enrichment of 22nt sRNAs produced via the DICER2 (DCL2) biogenesis pathway. However, the specific role of DICER2 -dependent 22nt sRNAs remains underexplored. We examined the EPRV -derived 22nt sRNAs in both wild-type and dcl2 tomato mutants to elucidate the role of these DCL2 -dependent 22nt sRNAs in plant immunity, especially concerning hybrid vigour. Utilizing the long-read tomato genome and bioinformatics, we analysed regions across four EPRV subtypes where 22nt sRNAs are mapped, revealing variations in the consensus sequences of the EPRV subgenera. These findings offer insights into the role of DICER2 and EPRV -derived 22nt sRNAs in plant viral immunity, particularly in hybrid plants, providing a foundation for further research.

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This talk is part of the Department Of Plant Sciences Seminar Series series.

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• Department Of Plant Sciences Seminar Series
• Department of Plant Sciences, Large Lecture Theatre

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