University of Cambridge > Talks.cam > MRC LMB Seminar list > Kendrew Lecture 2022: Chemogenetic and optogenetic technologies for probing molecular and cellular networks

Kendrew Lecture 2022: Chemogenetic and optogenetic technologies for probing molecular and cellular networks

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Where a protein is localized in the cell exerts tremendous influence over its function, interaction partners, dynamics, and modifications. Enzyme-catalyzed proximity labeling (PL) has emerged as a powerful and generalizable method to map the locations and interactions of endogenous proteins in the context of living cells, applicable to even membraneless organelles and transient interactions that are inaccessible to traditional methods such as affinity purification. I will give a brief account of PL method development, including directed evolution of the PL enzymes APEX and TurboID, and then describe new efforts to extend PL to RNA and proteome trafficking. In the second part of the talk I will describe recent work on the development of scalable, single-cell molecular recorders of past cellular events, and the application of these recorders to neuroscience and mitochondrial biology.

This talk is part of the MRC LMB Seminar list series.

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