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Direct Selection for Antibodies that Control Cell Fates from Intracellular Combinatorial Antibody Libraries

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In this talk we will describe the use of combinatorial antibody libraries to endow cells with new binding energy landscapes for the purpose of regulating their phenotypes. Antibodies that are expressed in cells infected with a lentiviral combinatorial antibody library are selected directly for function rather than only for binding. Many different agonist antibodies were selected using this method including TPO , EPO, G-CSF, integrin, and neurogenesis phenocopies.. The most powerful format is an autocrine based selection system.

This talk is part of the MRC LMB Seminar Series series.

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