Edit-R CRISPR-Cas9 tools for functional genomics discovery

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• Dr Doris Beylkin, Dharmacon
• Friday 24 March 2017, 13:00-14:00
• CRUK CI Lecture Theatre.

If you have a question about this talk, please contact Alasdair.Russell.

At GE-Dharmacon we have been developing a wide range of capabilities for CRISPR -CAS9. This has involved the development of an algorithm for the design of highly functional Crispr sequences and the subsequent development of products to enable knockout and knockin work.

One of the main areas in which we have made significant advances has been in the development of arrayed crispr screening. For this we have designed a system that employs synthetic crRNA arrayed into 96 or 384 well plates, enabling screening to be performed using assays that are not suitable for a pooled based crispr screen.

In the seminar we will discuss:

- Development of the algorithm for design of highly penetrant crRNAs. - The different applications of arrayed and pooled crispr screening. - Examples where arrayed screening has been employed successfully, two examples of internal screens performed by Dharmacon scientists and two examples of screens performed by customers.

This talk is part of the CRUK CI Seminars series.

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• CRUK CI Lecture Theatre
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