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Phage Sf6 ejection mechanisms

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  • UserProfessor Kristin N. Parent, Michigan State University, East Lansing, United States
  • ClockTuesday 17 January 2017, 15:30-16:00
  • HouseOnline.

If you have a question about this talk, please contact Ilana Spilka.

Outer membrane proteins A and C mediate Sf6 infection by dramatically increasing the rate and efficiency of genome ejection. I will describe a combination of in vivo studies with omp null mutants of Shigella flexneri, including classic phage assays and time-lapse fluorescence microscopy to monitor genome ejection at the single virion level, and cryo-electron tomography of phage “infecting” outer membrane vesicles. In addition, mutagenesis studies have demonstrated key regions in OmpA that mediate infection. We conclude that Sf6 phage entry utilizes either outer membrane proteins A or C, with outer membrane protein A being the preferred receptor.

This talk is part of the Bacteriophage 2017 series.

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