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Detection of phage φ1207.3 in Streptococcus pneumoniae by immunoassays targeting the major capsid protein

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  • UserDr. Francesco Santoro, University of Siena, LAMMB – Dept. of Medical Biotechnologies, Siena, Italy
  • ClockTuesday 17 January 2017, 08:30-09:00
  • HouseOnline.

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Dr. Francesco Santoro, University of Siena, LAMMB - Dept of Medical Biotechnologies, Siena, Italy Φ1207.3 is a prophage found in a clinical strain of Streptococcus pyogenes which carries macrolide resistance genes. We previously transferred the phage in Streptococcus pneumoniae and showed that it is able to produce mature and complete phage particles. We evaluated the presence of phage particles associated to the host in a bacterial culture of S. pneumoniae using polyclonal antibodies directed against the phage major capsid protein. Φ1207.3 major capsid protein was detected both inside and on the surface of the pneumococcus by western-blot, flow cytometry, immunofluorescence and immuno TEM .

This talk is part of the Bacteriophage 2017 series.

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