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Panoramic Light Sheet Microscopy

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Panoramic Light Sheet Microscopy, Gopi Shah, Light Microscopy, CRUK Cambridge Institute and Jan Huisken Lab, MPI -CBG, Dreden

A monthly lecture series on imaging techniques, analysis tools, imaging applications and developments across disciplines organised by Stefanie Reichelt (CRUK CI), Alex Sossick (Gurdon) and Kevin O’Holleran (CAIK)

The ever-increasing speed and resolution of modern microscopes make the storage and post-processing of images challenging. We developed a 4-lens light sheet microscope combined with real-time image-processing engine that projects 3D image volume onto 2D map, reducing the data generated as well as providing a panoramic view of the sample.

In this talk, will be discussed the multi-dimensional imaging approach, integrating whole-embrio life imaging, genetic information and analysis of the ensemble of specimen to understand how large-scale tissue movements shape the early zebrafish embrio.

www.lightmicroscopy.cruk.cam.ac.uk

This talk is part of the Synthetic Biology Strategic Research Initiative series.

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