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“Non Linear Microscopy: from morpho-functional imaging and manipulation to structural dynamics"

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Some non laser linear imaging techniques will be described, ranging from two photon, lifetime and multi-spectral, up to the second harmonic generation microscopy, illustrating some experiments exploiting the capabilities of such techniques. An interactive approach to perform cell biology will be described with some optical manipulation experiments based on non linear interactions (nano-surgery, optical knock out, trapping) and coupled to imaging operation. Also, the use of second harmonic generation imaging will be demonstrated as a tool to address information on structural dynamics in living cells at the atomic level, well below the diffraction limit of the microscope.

Information from structural dynamics in living cell to the order at a larger scale, such as in issues, will be demonstrated also with the use of SHG in tissues such as skin or cornea. Multi spectral imaging will be shown as an important tool for also for cancer detection. In the frame of tissue imaging, functional imaging of brain will be described in living animals, in interacting experiments with nano-surgery and recorded response in plasticity of the central nervous system. Also, new techniques of non linear imaging, reaching more than 2000 frames per second based on fast scanning methods or on special multi-beams configuration for electrical signal detections in neurons will be described.

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